Center for Infectious Disease and Vector Research

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informational Seminar: Digital Droplet PCR (Thurs Sept 15) 2-330PM

Thursday, September 15, 2016
23:30 p.m.


Keen Hall
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Biorad  and IIGB will present a technical seminar that focuses on Droplet Digital™ PCR applications in support of the QX200 ddPCR™ system.


Thursday September 15, 2:00 PM - 3:30 PM University of California, Riverside Genomics Building Auditorium 1102A


SPEAKER: Carina Emery, Field Application Scientist, Bio-Rad Laboratories


Droplet Digital PCR: An Overview of the Technology and Its Applications Digital PCR is a breakthrough technology that provides ultrasensitive and absolutenucleic acid quantification. This technique is particularly useful for low abundancetargets, targets in complex backgrounds, allelic variants (SNPs) and for monitoring ofsubtle changes in target levels. In the Bio-Rad Labratories’ Droplet Digital™ PCR (ddPCR™) System, each PCR sample is partitioned into a large number of microscopic droplets prior to amplification.

Each droplet is an individual PCR reaction. After end-point amplification, fluorescence is detected in the droplets in which target sequence was amplified. These droplets are scored as positive. Droplets not containing the target sequence show little or no fluorescence and are scored as negative. Using the Poisson distribution law, the fraction of positive droplets is converted to the number of molecules in the starting sample, without the need for standard curves (absolute quantification).

Applications for digital PCR cover different areas of biology. The following describes some of the most popular fields of application: Liquid Biopsy, Copy Number Variation (CNV), Rare Sequence Detection, Gene Expression, Single-Cell Analysis, Pathogen Detection and Microbiome Analysis, and Next-Generation Sequencing (NGS).

Additional Information:

Open to:

General Public




Institute for Integrative Genome Biology

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