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Seminar:
CEPCEB Seminar Dr. Pal Maliga

Friday, January 13, 2017
Noon1 p.m.

Location:

Genomics Building
Parking Information

Description:

 


 

Dear Faculty, Postdocs, Students, and Friends:

You are cordially invited to a seminar presented by

 



Pal Maliga

Rutgers University

Waksman Institute of Microbiology

and Department of Plant Biology

 

Title:

“CELL-TO-CELL MOVEMENT OF PLASTIDS AND MITOCHONDRIA IN PLANTS”


DATE: Friday, January 13, 2017

 LOCATION: Genomics Auditorium, RM 1102A

Time:12:10pm

 

Host: Meng Chen

 

Abstract:

 

To detect organelle movement between cells, we grafted two different species of tobacco, Nicotiana tabacum and Nicotiana sylvestris. We initiated tissue culture from sliced graft junctions and selected for clonal lines in which gentamycin resistance encoded in the N. tabacum nucleus was combined with spectinomycin resistance encoded in N. sylvestris plastids. We obtained evidence for cell-to-cell movement of the entire 161-kb plastid genome in these plants, most likely in intact plastids 1. In some of the clones, mitochondrial DNA movement was also detected in regenerated plants by restoration of pollen fertility in the cytoplasmic male sterile (CMS) graft partner 2. Homologous recombination yielded fertile and sterile mitochondrial genomes due to recombination at alternative sites, linking CMS to a unique open reading frame in CMS mitochondria. Cell-to-cell movement of plastids and mitochondria supports the universality of intercellular organelle trafficking and enables modification of the mitochondrial genome by DNA transmitted from a sexually incompatible species. A mitochondrial trait of commercial interest could be CMS in species, such as tomato, where CMS currently does not exist.

Additional Information:

Open to:

General Public

Admission:

Free

Sponsor:

Institute for Integrative Genome Biology


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