UCR

Center for Infectious Disease and Vector Research



Events Calendar


Lecture:
Applied Stem Cell Gene Editing Semina

Tuesday, February 24, 2015
11 a.m.Noon

Location:

Genomics Building , 1102A
Parking Information

Description:

Dear IIGB Genomics Core Users,

 

IIGB and the Stem Cell Center are hosting an informational seminar by Applied StemCell, Ins about genome editing using CRISPR in human and animal models. This technology is being widely applied to many organisms.

 

EVENT

Applied Stem Cell Gene Editing Seminar

 

SEMINAR TITLE

Genome Editing Using CRISPR TARGATT in Human Cell Lines and Animal Models

 

DATE: Tuesday February 24

TIME:11AM to Noon (plus Q&A time)

LOCATION: Genomics Building Auditorium 1102A

 

SPEAKER:

Ruby Yanru Chen-Tsai, PhD, CSO, Applied StemCell, Inc.

 

COFFEE AND REFRESHMENTS WILL BE SERVED!!

 

SUMMARY

The ability to genetically manipulate the mammalian genome has provided a powerful tool for the study of gene function, bioproduction and gene/cell therapy. For example, gene editing/correction in patient-specific induced pluripotent stem cells (iPSCs) offers one of the most promising approaches for personalized therapy in regenerative medicine. CRISPR/Cas9 has been widely used as an effective gene editing tool in the past two years for site-specific gene modification including insertion, deletion and nucleotide replacement. CRISPR works most effciently in gene knockout through the non-homologous end joining (NHEJ) pathway. However for DNA knockin, the efficiency of CRISPR mediated homology-directed recombination (HDR) remains low, in most cases below 1% without enrichment by drug selection or FACS sorting. In comparison, TARGATT™ site-specific gene insertion technology, an integrase based method, allows large DNA fragment insertion (up to 22kb) with high efficiency (up to 40%). We will discuss both the CRISPR and TARGATT™ technologies and their applications in cell line and animal model generation. We will also discuss our new research on increasing CRISPR DNA knock-in efficiency using small molecules.

 

Hope to see you there!

 

Your IIGB Genomics Core

Additional Information:

Open to:

General Public

Admission:

Free

Sponsor:

Institute for Integrative Genome Biology


More Information

General Campus Information

University of California, Riverside
900 University Ave.
Riverside, CA 92521
Tel: (951) 827-1012

Career OpportunitiesUCR Libraries
Campus StatusMaps and Directions

Department Information

Institute of Integrative Genomics Biology
2150 Batchelor Hall

Tel: (951) 827-7177

Footer